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Tuesday, November 6, 2007
The Objectives
These are all the objectives of the experiments.(Although at each day we already put the objectives)These are for people who ONLY want to know our objectives. =) So that you will not have trouble going to every different experiment to see the objectives.
Overall Objective:
To carry out large scale of fermentation to obtain pure isolated product
Experiment No. 1 Familiarisation with the Bioreactor and its Operation
Overall Objective:
To carry out large scale of fermentation to obtain pure isolated product
Experiment No. 1 Familiarisation with the Bioreactor and its Operation
- To familiarize students with the parts and components of microbial and mammalian Bioreactors
- To introduce the basic operation procedure of a bioreactor.
Experiment No. 2 Equipment, Media and Seed Culture Preparation
- To describe the steps to prepare a bioreactor
- To prepare the media for seed culture and scale-up fermentation
- To prepare seed culture for scale-up fermentation
Experiment No. 3 Inoculation, Fermentation and Monitoring
- To carry out scale-up fermentation process to increase the yield of desired protein product (Green florescent protein in this case).
- To monitor cell growth and product formation through manual sampling and computer data logging.
Experiment No. 4 Isolation and Purification of Product
- To perform cell lysis to isolate the desired protein product (Green florescent protein)
- To perform Gel Permeation chromatography to purify the extract
7:40 AM
posted by edyna @ 7:40 AM
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